Israel's Foreign Ministry lodged a protest after Jordan's Information Minister and Government Spokeswoman Jumana Ghunaimat was seen stepping on an Israeli flag.
Ghunaimat had trampled on the Israeli flag during a visit to Amman's trade union headquarters, where a Jordanian government meeting was held.
The trade union headquarters in Jordan stuck an Israeli flag at the entrance to its offices, inviting passersby to step on it on their way inside, a deep insult in the Arab world.
Prime Minister Omar al-Razaz entered the road through a side entrance, thus avoiding the Israeli flag.
The Jordanian Ambassador to Israel was summoned to a clarification meeting on Sunday.