Border Police officers from the Jerusalem Envelope unit and crossing security personnel on Sunday night stopped a private vehicle at the Tunnels Crossing on the route from Gush Etzion to Jerusalem for inspection.
During the search, officers opened the vehicle's trunk and discovered a donkey inside with its legs bound.
The driver, a 28-year-old resident of Beitar Illit, told officers the donkey was unwell and that he was transporting it to a farm in Jerusalem's Neve Yaakov neighborhood. Officers detained him for questioning.
The donkey was transferred to an animal welfare organization for treatment.
A Border Police statement stressed that officers "will continue to work determinedly to enforce the law and act in any incident where there is a suspicion of harm to animals or of violation of the law."